MultiPLX: automatic grouping and evaluation of PCR primers

MultiPLX: automatic grouping and evaluation of PCR primers

UNLABELLED MultiPLX is a new program for automatic grouping of PCR primers. It can use many different parameters to estimate the compatibility of primers, such as primer-primer interactions, primer-product interactions, difference in melting temperatures, difference in product length and the risk of generating alternative products from the template. A unique feature of the MultiPLX is the abili...

Evaluation of PCR primers for cytomegalovirus detection.

We read with great interest the article by Mendez et al. (2) published in a recent issue evaluating different PCR primers for cytomegalovirus (CMV) detection. As CMV PCR becomes a frequently used assay in the management of immunocompromised patients, such as transplant recipients and human immunodeficiency virus-infected individuals, an increasing number of laboratories are establishing in-hous...

MFEprimer: multiple factor evaluation of the specificity of PCR primers

SUMMARY We developed a program named MFEprimer for evaluating the specificity of PCR primers based on multiple factors, including sequence similarity, stability at the 3'-end of the primer, melting temperature, GC content and number of binding sites between the primer and DNA templates. MFEprimer can help the user to select more suitable primers before running either standard or multiplex PCR r...

Designing PCR Primers Painlessly†

PCR with arbitrary primers